RT-PCR protocols

Contributor(s): King, Nicola, DrMaterial type: TextTextLanguage: English Series: Springer protocolsPublisher: New York : Humana Press, ©2010Edition: 2nd ed. / edited by Nicola KingDescription: xi, 341 p. : ill. (certaines en coul.) ; 27 cmISBN: 9781607616283; 1607616289Subject(s): Réaction en chaîne de la polymérase -- Manuels de laboratoire | BIOLOGÍA MOLECULAR -- MÉTODOSDDC classification: 574.8 Online resources: table of contents
Contents:
Single cell RT-PCR on mouse embryos : a general approach for developmental biology / Wolfgang Mann and Thomas Haaf -- Poly(A) cDNA real-time PCR for indicator gene measurement in cancer / Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers -- Transcriptome profiling of host-microbe interactions by differential display RT-PCR / Leong Wai Fook and Vincent T.K. Chow -- Quantitative RT-PCR methods for mature microRNA expression analysis / Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson -- Detection of influenza A virus neuraminidase and PB2 gene segments by one step reverse transcription polymerase chain reaction / Alejandra Castillo Alvarez ... [et al.] -- Detection and identification of CD46 splicing isoforms by nested RT-PCR / Anita Szalmás ... [et al.] -- Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR / Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq -- Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR / Ana Silvia González-Reiche ... [et al.] -- Detection of antisense RNA transcripts by strand-specific RT-PCR / Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville -- RT-PCR amplification and cloning of large viral sequences / Xiaofeng Fan and Adrian M. Di Bisceglie -- One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification / Cristina Hartshorn and Lawrence J. Wangh -- Changes in gene expression of caveolin-1 after inflammatory pain using quantitative real-time PCR / Fiza Rashid-Doubell -- Real-time quantitative reverse transcriptase polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye -- The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes / Nicola King -- The renaissance of competitive PCR as an accurate tool for precise nucleic acid quantification / Lorena Zentilin and Mauro Giacca -- Skeletal muscle RNA extraction in preparation for RT-PCR / Janelle P. Mollica -- Reverse transcription of the ribonucleic acid : the first step in RT-PCR assay / Fadia Haddad and Kenneth M. Baldwin -- Primer design for RT-PCR / Kelvin Li and Anushka Brownley -- Hot start PCR / Natasha Paul, Jonathan Shum, and Tony Le -- Real-time RT-PCR for automated detection of HIV-1 RNA during blood donor screening / Jens Müller.
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Item type Current location Collection Call number Copy number Status Date due Barcode Item holds
Libros / General Libros / General Biblioteca Central UDES- Bucaramanga
Colección General 574.8 P768 (Browse shelf) Ej. 1 Available 028020
Total holds: 0

Comprend des réf. bibliogr. et un index.

Single cell RT-PCR on mouse embryos : a general approach for developmental biology / Wolfgang Mann and Thomas Haaf -- Poly(A) cDNA real-time PCR for indicator gene measurement in cancer / Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers -- Transcriptome profiling of host-microbe interactions by differential display RT-PCR / Leong Wai Fook and Vincent T.K. Chow -- Quantitative RT-PCR methods for mature microRNA expression analysis / Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson -- Detection of influenza A virus neuraminidase and PB2 gene segments by one step reverse transcription polymerase chain reaction / Alejandra Castillo Alvarez ... [et al.] -- Detection and identification of CD46 splicing isoforms by nested RT-PCR / Anita Szalmás ... [et al.] -- Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR / Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq -- Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR / Ana Silvia González-Reiche ... [et al.] -- Detection of antisense RNA transcripts by strand-specific RT-PCR / Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville -- RT-PCR amplification and cloning of large viral sequences / Xiaofeng Fan and Adrian M. Di Bisceglie -- One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification / Cristina Hartshorn and Lawrence J. Wangh -- Changes in gene expression of caveolin-1 after inflammatory pain using quantitative real-time PCR / Fiza Rashid-Doubell -- Real-time quantitative reverse transcriptase polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye -- The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes / Nicola King -- The renaissance of competitive PCR as an accurate tool for precise nucleic acid quantification / Lorena Zentilin and Mauro Giacca -- Skeletal muscle RNA extraction in preparation for RT-PCR / Janelle P. Mollica -- Reverse transcription of the ribonucleic acid : the first step in RT-PCR assay / Fadia Haddad and Kenneth M. Baldwin -- Primer design for RT-PCR / Kelvin Li and Anushka Brownley -- Hot start PCR / Natasha Paul, Jonathan Shum, and Tony Le -- Real-time RT-PCR for automated detection of HIV-1 RNA during blood donor screening / Jens Müller.

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